期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 407, 期 27, 页码 8353-8362出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-015-9020-8
关键词
Surface-enhanced Raman scattering; Anti-CCP; Rheumatoid arthritis; Immunoassay; Early diagnosis
资金
- National Research Foundation of Korea [2008-0061856, 2009-00426, 2013R1A1A2063269]
- Nano Materials Technology Development Program through the National Research Foundation of Korea
- Ministry of Education, Science and Technology [2012M3A7B4035288]
- National Research Foundation of Korea [2013R1A1A2063269, 2008-0061856] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We assessed the clinical feasibility of conducting immunoassays based on surface-enhanced Raman scattering (SERS) in the early diagnosis of rheumatoid arthritis (RA). An autoantibody against citrullinated peptide (anti-CCP) was used as a biomarker, magnetic beads conjugated with CCP were used as substrates, and the SERS nanotags were comprised of anti-human IgG-conjugated hollow gold nanospheres (HGNs). We were able to determine the anti-CCP serum levels successfully by observing the distinctive Raman intensities corresponding to the SERS nanotags. At high concentrations of anti-CCP (> 25 U/mL), the results obtained from the SERS assay confirmed those obtained via an ELISA-based assay. Nevertheless, quantitation via our SERS-based assay is significantly more accurate at low concentrations (< 25 U/mL). In this study, we compared the results of an anti-CCP assay of 74 clinical blood samples obtained from the SERS-based assay to that of a commercial ELISA kit. The results of the anti-CCP-positive group (n = 31, > 25 U/mL) revealed a good correlation between the ELISA and SERS-based assays. However, in the anti-CCP-negative group (n = 43, < 25 U/mL), the SERS-based assay was shown to be more reproducible. Accordingly, we suggest that SERS-based assays are novel and potentially useful tools in the early diagnosis of RA.
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