期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 18, 期 10, 页码 3107-3111出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2007.10.104
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资金
- NIDDK NIH HHS [T32 DK007233-32, T32 DK007233, 2T32DK07233] Funding Source: Medline
Mass spectrometry (MS) is an important tool for studying non-ribosomal peptide, polyketide, and fatty acid biosynthesis. Here we describe a new approach using multi-stage tandem MS on a common ion trap instrument to obtain high-resolution measurements of the masses of substrates and intermediates bound to phosphopantetheinylated ( holo) carrier proteins. In particular, we report the chemical formulas of 12 diagnostic MS(3) fragments of the phosphopantetheine moiety ejected from holo carrier proteins during MS(2). We demonstrate our method by observing the formation of holo-AcpC, a putative acyl carrier protein from Streptococcus agalactiae. Published by Elsevier Ltd.
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