期刊
ENDOCRINOLOGY
卷 162, 期 11, 页码 -出版社
ENDOCRINE SOC
DOI: 10.1210/endocr/bqab161
关键词
Kisspeptin; Kiss1; GnRH; progesterone; LH surge; positive feedback; ovulation; AVPV; RP3V
资金
- National Institutes of Health (NIH) [R01 HD090161, R01 HD100580, R01 HD042635, F32 HD097965]
- National Institute of Child Health and Human Development (NICHD) [P50 HD012303, R24 HD1020614]
Kisspeptin plays a crucial role in regulating reproduction by activating gonadotropin-releasing hormone neurons. Progesterone's action in AVPV kisspeptin neurons is essential for the proper induction of E-2-induced LH surges.
Kisspeptin, encoded by Kiss1, stimulates gonadotropin-releasing hormone neurons to govern reproduction. In female rodents, estrogen-sensitive kisspeptin neurons in the rostral anteroventral periventricular (AVPV) hypothalamus are thought to mediate estradiol (E-2)-induced positive feedback induction of the preovulatory luteinizing hormone (LH) surge. AVPV kisspeptin neurons coexpress estrogen and progesterone receptors (PGRs) and are activated during the LH surge. While E-2 effects on kisspeptin neurons have been well studied, progesterone's regulation of kisspeptin neurons is less understood. Using transgenic mice lacking PGR exclusively in kisspeptin cells (termed KissPRKOs), we previously demonstrated that progesterone action specifically in kisspeptin cells is essential for ovulation and normal fertility. Unlike control females, KissPRKO females did not generate proper LH surges, indicating that PGR signaling in kisspeptin cells is required for positive feedback. However, because PGR was knocked out from all kisspeptin neurons in the brain, that study was unable to determine the specific kisspeptin population mediating PGR action on the LH surge. Here, we used targeted Cre-mediated adeno-associated virus (AAV) technology to reintroduce PGR selectively into AVPV kisspeptin neurons of adult KissPRKO females, and tested whether this rescues occurrence of the LH surge. We found that targeted upregulation of PGR in kisspeptin neurons exclusively in the AVPV is sufficient to restore proper E-2-induced LH surges in KissPRKO females, suggesting that this specific kisspeptin population is a key target of the necessary progesterone action for the surge. These findings further highlight the critical importance of progesterone signaling, along with E-2 signaling, in the positive feedback induction of LH surges and ovulation.
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