期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 22, 期 20, 页码 5692-5696出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2014.05.074
关键词
P450 peroxygenases; mNADHs; In situ H2O2 generation; Hydrogen peroxide shunt; Oxyfunctionalization
资金
- Marie Curie IEF 'BIOTRAINS' and 'BIOMIMIC' - European Union through the 7th Framework People Programme [238531, 327647]
An in situ H2O2 generation approach to promote P450 peroxygenases catalysis was developed through the use of the nicotinamide cofactor analogue 1-benzyl-1,4-dihydronicotinamide (BNAH) and flavin mononucleotide (FMN). Final productivity could be enhanced due to higher enzyme stability at low H2O2 concentrations. The H2O2 generation represented the rate-limiting step, however it could be easily controlled by varying both FMN and BNAH concentrations. Further characterization can result in an optimized ratio of FMN/BNAH/O(2)(/)biocatalyst enabling high reaction rates while minimizing H2O2-related inactivation of the enzyme. (C) 2014 Elsevier Ltd. All rights reserved.
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