4.1 Article

Near-complete genome sequence and seed transmission evaluation of Physalis rugose mosaic virus from southern Brazil

期刊

CIENCIA RURAL
卷 51, 期 4, 页码 -

出版社

UNIV FEDERAL SANTA MARIA
DOI: 10.1590/0103-8478cr20200702

关键词

characterization; PhyRMV; Sobemovirus

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资金

  1. Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brasil [001]
  2. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [CNPq -437059/2018-9]
  3. Fundacao de Amparo a Pesquisa e Inovacao do Estado de Santa Catarina

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The study characterized the Physalis rugose mosaic virus (PhyRMV) associated with Physalis peruviana and evaluated its transmission through seeds, finding it unlikely to be transmitted through physalis seeds.
Physalis rugose mosaic virus (PhyRMV) causes severe damage to Physalis peruviana L., affecting vegetative parameters, fruit quantity and quality. The aim of this study was to perform a molecular characterization of PhyRMV associated with P. peruviana from commercial fields in the municipality of Lages, Santa Catarina State, Southern Brazil, and to evaluate its transmission by seeds. Plants displaying mosaic. dwarfism. and leaf malformation symptoms were collected from P. peruviana. Double-stranded RNA was extracted and submitted to cDNA library synthesis and high-throughput sequencing (HTS). For the virus transmission assay, seeds from PhyRMV-infected plants were used. and viral infection in seedlings was verified using symptomatic and molecular diagnosis. PhyRMV RNA has 4162 nucleotides (nts) and a genomic organization similar to that of other sobemoviruses and shares 97% nt identity with the previously characterized PhyRMV Piracicaba isolate Results indicated the unlikely transmission of PhyRMV by physalis seeds.

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