期刊
GENETICS
卷 217, 期 1, 页码 -出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/genetics/iyaa001
关键词
DNA replication; Sld3; Esa1; silencing; origin firing
资金
- Uehara Memorial Foundation
- Institute for Fermentation, Osaka
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science [25131721]
DNA replication in eukaryotes involves a multi-step process with key reactions including helicase loading, activation, and nascent DNA synthesis. While chromatin regulatory factors have been studied in licensing and elongation, their role in the firing reaction remains unclear. In yeast, overexpression of 3-7-45 leads to untimely activation of replication origins, with interactions between Sld3 and Esa1 playing a crucial role in this process. These results highlight the novel regulatory role of Sld3-Esa1 interaction in DNA replication.
DNA replication in eukaryotes is a multi-step process that consists of three main reactions: helicase loading (licensing), helicase activation (firing), and nascent DNA synthesis (elongation). Although the contributions of some chromatin regulatory factors in the licensing and elongation reaction have been determined, their functions in the firing reaction remain elusive. In the budding yeast Saccharomyces cerevisiae, Sld3, Sld7, and Cdc45 (3-7-45) are rate-limiting in the firing reaction and simultaneous overexpression of 3-7-45 causes untimely activation of late and dormant replication origins. Here, we found that 3-7-45 overexpression not only activated dormant origins in the silenced locus, HML alpha, but also exerted an anti-silencing effect at this locus. For these, interaction between Sld3 and Esa1, a conserved histone acetyltransferase, was responsible. Moreover, the Sld3-Esa1 interaction was required for the untimely activation of late origins. These results reveal the Sld3-Esa1 interaction as a novel level of regulation in the firing reaction.
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