Photosynthetic capacity of male and female Hippophae rhamnoides plants along an elevation gradient in eastern Qinghai-Tibetan Plateau, China

Elevational variations in the growing environment and sex differences in individuals drive the diversification of photosynthetic capacity of plants. However, photosynthetic response of dioecious plants to elevation gradients and the mechanisms that cause these responses are poorly understood. We measured foliar gas exchange, chlorophyll fluorescence and nitrogen allocations of male and female Seabuckthorn (Hippophae rhamnoides L.) at the elevation of 1900-3700 m above sea level (a.s.l.) on the eastern Qinghai-Tibetan Plateau, China. Male and female plants showed increased leaf photosynthetic capacity at higher elevation generally with no sex-specific difference. Photosynthetic photon flux density-saturated photosynthesis (A(sat)) was limited mostly by diffusional components (77 +/- 1%), whereas biochemical components contributed minor limitations (22 +/- 1%). Mesophyll conductance (g(m)) played an essential role in A(sat) variation, accounting for 40 +/- 2% of the total photosynthetic limitations and had a significant positive correlation with A(sat). Leaf nitrogen allocations to Rubisco (P-R) and bioenergetics (P-B) in the photosynthetic apparatus were major drivers for variations in photosynthetic nitrogen-use efficiency. The increase of these resource uptake capacities enables H. rhamnoides to maintain a high level of carbon assimilation and function efficiently to cope with the harsh conditions and shorter growing season at higher elevation.

Automated summary

Elevational variations and sex differences drive the diversification of photosynthetic capacity in plants, but the mechanisms are poorly understood. Photosynthetic capacity in Seabuckthorn plants generally increases with elevation, with mesophyll conductance playing a key role. Leaf nitrogen allocations are crucial for photosynthetic nitrogen-use efficiency.