期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 21, 期 20, 页码 6119-6123出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2013.05.030
关键词
microRNA/RNAi pathway; Reporter system; High-throughput screening; Small molecule; Chemical biology approach; Enhancer; Inhibitor
资金
- NIA NIH HHS [P50 AG025688] Funding Source: Medline
- NIMH NIH HHS [R01 MH076090] Funding Source: Medline
- NINDS NIH HHS [R01 NS051630] Funding Source: Medline
MicroRNA (miRNA)/RNA interference (RNAi) is recognized as one of the most important mechanisms regulating gene expression at the posttranscriptional level in eukaryotic cells. The main components within the miRNA/RNAi pathway are now known and well characterized, but studies on the molecular mechanisms that regulate the activity of the miRNA/RNAi pathway are just beginning to emerge. High-throughput reporter assays have been developed to monitor the activity of the miRNA/RNAi pathway and applied in a proof-of-concept pilot screening, which has led to the identification of some inhibitors and activators that either generally or specifically regulate the activity of the miRNA/RNAi pathway. In addition, combined with multidisciplinary approaches like proteomics, biochemistry, and genetics, some protein co-factors were found to play important roles in the regulation of the miRNA/RNAi pathway. Herein we highlight the high-throughput reporter assays developed in recent years and the resulting discovery of miRNA/RNAi enhancers and inhibitors. (C) 2013 Elsevier Ltd. All rights reserved.
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