期刊
MOLECULAR & CELLULAR PROTEOMICS
卷 20, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.mcpro.2021.100159
关键词
-
资金
- Swedish Research Council [2017-01330, 2016-01675, 2018-06156, 2018-02266]
- Karolinska Institutet [2020-02153, 2020-01554]
- Ake Wiberg Stiftelse [M20-0220]
- Ragnar Soderberg Foundation [M13/18]
This study used patient-derived data and in vitro experiments to investigate the relationship between metabolic pathways and the replication of the SARS-CoV-2 virus, finding that severe disease is characterized by increased plasma glucose levels. Immune phenotype analysis revealed altered expression patterns, and glycolysis and glutaminolysis were identified as essential for virus replication.
Viruses hijack host metabolic pathways for their replicative advantage. In this study, using patient-derived multiomics data and in vitro infection assays, we aimed to understand the role of key metabolic pathways that can regulate severe acute respiratory syndrome coronavirus-2 reproduction and their association with disease severity. We used multiomics platforms (targeted and untargeted proteomics and untar-geted metabolomics) on patient samples and cell-line models along with immune phenotyping of metabolite transporters in patient blood cells to understand viral -induced metabolic modulations. We also modulated key metabolic pathways that were identified using multiomics data to regulate the viral reproduction in vitro. Coronavirus disease 2019 disease severity was characterized by increased plasma glucose and mannose levels. Immune phenotyping identified altered expression patterns of car-bohydrate transporter, glucose transporter 1, in CD8(+) T cells, intermediate and nonclassical monocytes, and amino acid transporter, xCT, in classical, intermediate, and nonclassical monocytes. In in vitro lung epithelial cell (Calu-3) infection model, we found that glycolysis and glutaminolysis are essential for virus replication, and blocking these metabolic pathways caused significant reduction in virus production. Taken together, we therefore hypothesized that severe acute respiratory syndrome coronavirus-2 utilizes and rewires pathways governing central carbon metabolism leading to the efflux of toxic metabolites and associated with disease severity. Thus, the host metabolic perturbation could be an attractive strategy to limit the viral replication and disease severity.
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