期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 21, 期 6, 页码 1534-1538出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2012.07.024
关键词
Carbonic anhydrase; Sulfonamide; Alpha-class enzyme; Inhibitor; Sulfurihydrogenibium yellowstonense; Thermophilic bacteria
资金
- FP7 EU project (Metoxia)
- Accordo di Programma CNR-MSE
The alpha-carbonic anhydrase (CA, EC 4.2.1.1) from the newly discovered thermophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1 (SspCA) was investigated for its inhibition with a large series of sulfonamides and a sulfamate, the classical inhibitors of these zinc enzymes. SspCA showed an inhibition profile with these compounds very similar to that of the predominant human cytosolic isoform hCA II, and not to that of the bacterial alpha-CA from Helicobacter pylori. Some clinically used drugs such as acetazolamide, methazolamide, ethoxzolamide, dichlorophenamide, dorzolamide, brinzolamide, topiramate, celecoxib and sulthiame were low nanomolar SspCA/hCA II inhibitors (K(I)s in the range of 4.5-12.3 nM) whereas simple aromatic/heterocyclic sulfonamides were less effective, micromolar inhibitors. As this highly catalytically active and thermostable enzyme may show biotechnological applications, its inhibition studies may be relevant for designing on/off systems to control its activity. (C) 2012 Elsevier Ltd. All rights reserved.
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