3.8 Article

Humanized Culture Medium for Clinical-Grade Generation of Erythroid Cells from Umbilical Cord Blood CD34(+) Cells

期刊

ADVANCED PHARMACEUTICAL BULLETIN
卷 11, 期 2, 页码 335-342

出版社

TABRIZ UNIV MEDICAL SCIENCES & HEALTH SERVICES
DOI: 10.34172/apb.2021.031

关键词

Human Platelet Lysate; Fetal Bovine Serum; CD34+ Hematopoietic Stem Cells; Erythroid Differentiation

资金

  1. Immunology Research Center at Tabriz University of Medical Sciences, Iran [58826]

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The study found that platelet lysate (PL) can enhance the proliferation and differentiation of CD34(+) HSCs, suggesting it as a suitable alternative in culture medium and HSCs differentiation.
Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost. Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34(+) umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and glycophorin A expression levels were evaluated. Results: Maximum hematopoietic stem cells (HSCs) proliferation was observed on day 15 in PL-containing medium (99 +/- 17x10(3)-fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium. Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34(+) HSCs. PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation.

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