Synthetic biotinylated tetra beta(1 -> 5) galactofuranoside for in vitro aspergillosis diagnosis

The synthesis of a tetra beta(1 -> 5) galactofuranoside was achieved using a thioglycoside donor with a methyl tert-butyl phenyl thio leaving group. This tetrasaccharide was conjugated to biotin and validated as antigen with the monoclonal antibody used for clinical detection of Aspergillus fumigatus galactomannan on streptavidin-coated microplates. Then we have shown its ability to detect antibodies associated with A. fumigatus induced disease by using sera from patients with Allergic broncho-pulmonary aspergillosis (ABPA) and correlated the results of antibody detection with those gained with a commercially available diagnostic test. (C) 2010 Elsevier Ltd. All rights reserved.