期刊
CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM
卷 48, 期 1, 页码 50-60出版社
KOREAN SOC REPRODUCTIVE MEDICINE
DOI: 10.5653/cerm.2020.03972
关键词
GABA receptors; Intracytoplasmic sperm injection; Oligospermia; Semen analysis; Spermatozoa; Teratozoospermia
资金
- Thailand Research Fund (TRF)
- Naresuan University Research Fund
- Program, Thailand
The study found that mRNA levels of GABA receptors in sperm were significantly higher in OAT men compared to NOR men, while the difference between TER and NOR men was not significant. High levels of these receptors were correlated with poor sperm parameters and embryo quality after ICSI treatment.
Objective: This study investigated the mRNA expression of gamma-aminobutyric acid (GABA) receptors in the sperm of oligoasthenoteratozoospermic (OAT) and teratozoospermic (TER) men compared to normozoospermic (NOR) men, as well as the relationships between GABA receptor expression and sperm parameters, fertilization rate, and embryo quality. Methods: The mRNA expression of GABA A-a1 and GABA B-R2 receptors in sperm was examined using reverse transcription-polymerase chain reaction in three groups of patients: NOR (n=32), OAT (n=22), and TER (n=45). The fertilization rate and embryo quality were assessed in 35 patients undergoing intracytoplasmic sperm injection (ICSI; 10 NOR, 10 OAT, and 15 TER men). Results: OAT men had significantly higher mRNA expression of GABA A-a1 and GABA B-R2 receptors in sperm than NOR men; however, the difference between TER and NOR men was not significant. High levels of these receptors were significantly correlated with low sperm concentration, motility, and morphology, as well as the rate of good-quality embryos (GQEs) at the cleavage stage after ICSI. Patients whose female partners had a >50% GQE rate at the cleavage stage had significantly lower levels of GABA A-a1 receptor expression than those whose partners had a <= 50% GQE rate. Conclusion: Our findings indicate that mRNA levels of GABA receptors in human sperm are correlated with poor sperm quality and associated with embryo development after ICSI treatment. The GABA A-a1 receptor in sperm has a stronger relationship with embryo quality at the cleavage stage than the GABA B-R2 receptor.
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