期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 19, 期 1, 页码 440-447出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2010.11.009
关键词
Lignin; Lignin oxidation; Horseradish peroxidase; Layer by layer; Enzyme immobilisation; (PNMR)-P-31
Horseradish peroxidase (HRP) was chemically immobilised onto alumina particles and coated by polyelectrolytes layers, using the layer-by-layer technique. The reactivity of the immobilised enzyme was studied in the oxidative functionalisation of softwood milled wood and residual kraft lignins and found higher than the free enzyme. In order to investigate the chemical modifications in the lignin structure, quantitative P-31 NMR was used. The immobilised HRP showed a higher reactivity with respect to the native enzyme yielding extensive depolymerisation of lignin. (C) 2010 Elsevier Ltd. All rights reserved.
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