期刊
ACS OMEGA
卷 6, 期 51, 页码 35325-35333出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.1c04257
关键词
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资金
- Ministry of Education, Singapore [MOE2017-T2-2-064]
- MIT-SUTD International Design Centre
- Changi General HospitalSUTD, Singapore [CGH-SUTD-HTIF2019-001]
- SUTDZhejiang-University [SUTD-ZJU (VP) 201903]
- National Supercomputing Centre, Singapore [15001618]
- Ministry of Education
- SUTD
The M2E system is a nondestructive cell monitoring method that can monitor cell recovery long-term with high cell viability.
Long-term nondestructive monitoring of cells is of significant importance for understanding cell proliferation, cell signaling, cell death, and other processes. However, traditional monitoring methods are limited to a certain range of testing conditions and may reduce cell viability. Here, we present a microgap, multishot electroporation (M2E) system for monitoring cell recovery for up to similar to 2 h using similar to 5 V pulses and with excellent cell viability using a medium cell population. Electric field simulations reveal the bias-voltage- and gap-size-dependent electric field intensities in the M2E system. In addition to excellent transparency with low cell toxicity, the M2E system does not require specialized components, expensive materials, complicated fabrication processes, or cell manipulations; it just consists of a micrometer-sized pattern and a low-voltage square-wave generator. Ultimately, the M2E system can offer a long-term and nontoxic method of cell monitoring.
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