期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 18, 期 4, 页码 1583-1590出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2010.01.004
关键词
Ghrelin; Des-acyl ghrelin; GHS-R1a; NMR spectroscopy
资金
- FIS
- Instituto de Salud Carlos III
- Ministerio de Ciencia e Innovacion [PI050382, PI060239, PI060705]
- Xunta de Galicia [PGIDIT05BTF20802PR, PGIDIT06PXIB918360PR]
The study of the interaction of ghrelin (1), the endogenous ligand for the GH secretagogues receptor (GHS-R1a), and des-acyl ghrelin (2) with the GHS-R1a by NMR using living cells is presented, using GHS-R1a stably transfected cell lines (CHO and HEK 293) and wild type cells. Therefore, the interaction of 1 and 2 with the GHS-R1a receptor has been performed using quasi-physiological conditions. Ghrelin (1), showed a higher number of residues affected by chemical shift perturbation (CSP) or chemical shift exchange (CSE) effects: Ser3, Phe4, Leu5, Val12, Gln13/Gln14, Lys16/Lys19, Glu17 and Lys24 were much more affected in 1 than in des-acyl ghrelin (2). The chemical shift index CSI values indicated the presence of a possible a-helical region between Glu8 and Lys20 for ghrelin (1). After analysing the NMR data, two possible structures have arisen, which present different proline rotamers: the EEZE and the EZEZ conformers, at positions Pro7, Pro21, Pro22 and Pro27, respectively, keeping a left-handed a-helix from Glu8 to Lys20. These experimental evidences might imply that the GHS-R1a receptor is acting as a prolylcis/trans isomerase. (C) 2010 Elsevier Ltd. All rights reserved.
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