期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 18, 期 1, 页码 267-273出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2009.10.060
关键词
Glucosidases; Proteomics probes; Photolabeling
资金
- Netherlands Proteomics Center (NPC)
Three different photoprobes were synthesized to label beta-glucosidases; one probe was based on glucose, two probes on the iminosugar deoxynojirimycin. The affinity of the probes for three different beta-glucosidases was determined. Furthermore, their labeling efficiencies, binding specificities through competition with deoxynojirimycin, and binding specificities in the presence of cell lysate, were evaluated. Especially one showed very high affinity towards non-lysosomal glucoceramidase (IC50 = 20 nM). (C) 2009 Elsevier Ltd. All rights reserved.
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