期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 17, 页码 9625-9632出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab716
关键词
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资金
- National Science Centre, Poland [2019/35/B/ST6/03074]
- Poznan University of Technology
- Institute of Bioorganic Chemistry, Polish Academy of Sciences
- Polish National Science Center
- Institute of Bioorganic Chemistry PAS
Computational methods for predicting RNA 3D structures have practical applications in molecular biology and medicine, highlighting the need to improve accuracy. The RNA-Puzzles initiative plays a significant role in collecting and evaluating computationally built RNAs, allowing for the analysis of prediction methods. This study focuses on entangled RNA fragments in predicted structures, proposing a classification based on topology and components.
Computational methods to predict RNA 3D structure have more and more practical applications in molecular biology and medicine. Therefore, it is crucial to intensify efforts to improve the accuracy and quality of predicted three-dimensional structures. A significant role in this is played by the RNA-Puzzles initiative that collects, evaluates, and shares RNAs built computationally within currently nearly 30 challenges. RNA-Puzzles datasets, subjected to multi-criteria analysis, allow revealing the strengths and weaknesses of computer prediction methods. Here, we study the issue of entangled RNA fragments in the predicted RNA 3D structure models. By entanglement, we mean an arrangement of two structural elements such that one of them passes through the other. We propose the classification of entanglements driven by their topology and components. It distinguishes two general classes, interlaces and lassos, and subclasses characterized by element types-loops, dinucleotide steps, open single-stranded fragments-and puncture multiplicity. Our computational pipeline for entanglement detection, applied for 1,017 non-redundant models from RNA-Puzzles, has shown the frequency of different entanglements and allowed identifying 138 structures with intersected assemblies.
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