期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 17, 页码 9870-9885出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab760
关键词
-
资金
- NIH [R35 GM126907]
- Cancer Center Support Grant NCI [P30CA008748]
Collisions between the replisome and RNA polymerases are the main obstacle to DNA replication. Utilization of mRNA transcripts as primers helps overcome co-directional collisions. The process involves synthesizing new primers downstream of the RNA polymerases to facilitate leading-strand synthesis during bypass.
Collisions between the replisome and RNA polymerases [RNAP(s)] are the main obstacle to DNA replication. These collisions can occur either head-on or co-directionally with respect to the direction of translocation of both complexes. Whereas head-on collisions require additional factors to be resolved, co-directional collisions are thought to be overcome by the replisome itself using the mRNA transcript as a primer. We show that mRNA takeover is utilized primarily after collisions with single RNAP complexes with short transcripts. Bypass of more complex transcription complexes requires the synthesis of a new primer downstream of the RNAP for the replisome to resume leading-strand synthesis. In both cases, bypass proceeds with displacement of the RNAP. Rep, Mfd, UvrD and RNase H can process the RNAP block and facilitate replisome bypass by promoting the formation of continuous leading strands. Bypass of co-directional RNAP(s) and/or R-loops is determined largely by the length of the obstacle that the replisome needs to traverse: R-loops are about equally as potent obstacles as RNAP arrays if they occupy the same length of the DNA template.
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