A successful case of assisted reproduction after contamination of the culture medium with Escherichia coli

INTRODUCTION: We report a case of successful pregnancy after in vitro fertilization culture (IVF) medium contaminated with Escherichia coli (E. coli). CASE REPORT: To remove E. coli, oocytes were isolated in a PVP-ICSI medium and then in a culture medium supplemented with gentamicin. We selected two embryos that were vitrified and frozen. In the next natural cycle, we performed the embryo transfer of 2 embryos. RESULTS: Only one pregnancy was uncomplicated until term and was completed by the live birth of a healthy baby. The child's subsequent development until the date of publication of this article is normal. CONCLUSIONS: In cases of E. coli contamination of the IVF culture medium, specific laboratory methods allow the procedure to be continued, and a healthy baby can be obtained.

Automated summary

This case report demonstrates a successful pregnancy after IVF culture medium was contaminated with E. coli. By utilizing specific laboratory methods to remove the contamination, a healthy baby was obtained.