Methylnigakinone content determination and geographical origin discrimination for P. quassioides via fluorescence fingerprint and principal component analyses

Picrasma quassioides is used as a bittersweet stomach medicine. Because it is a natural product obtained from various geographical regions, the production area is important when P. quassioides is used as a crude drug. Herein, we developed a method to determine the content of methylnigakinone, one of the major active in-gredients in P. quassioides, and a protocol for discriminating the geographical origin of this natural product using a fluorescence fingerprint analysis and principal component analysis (PCA). Because methylnigakinone is fluo-rescent (excitation wavelength: 352 nm, emission wavelength: 458 nm), the content of this molecule can be determined in the concentration range of 0.1-1 mu g/mL. The quantification results of methylnigakinone obtained using the developed method were similar to those obtained from an HPLC analysis. Furthermore, the PCA of the fluorescence fingerprint of P. quassioides produced a score plot with the three different geographical origins (Kyushu island (Japan), Shikoku island (Japan), and China) plotted in the regions. Thus, it was possible to discriminate the geographical origin of the P. quassioides samples. The developed method is simple, quick, and has a minimal environmental impact. Therefore, the developed method will be useful for confirming the origin of P. quassioides.

Automated summary

In this study, a method using fluorescence fingerprint analysis and principal component analysis was developed to discriminate the geographical origin of Picrasma quassioides. The content of methylnigakinone in P. quassioides was successfully determined using this method. The results suggest that the developed method is simple, quick, and of great importance for confirming the origin of P. quassioides.