期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 16, 期 17, 页码 8287-8293出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2008.05.042
关键词
pyrene; fluorescence; ODN; size discrimination
资金
- New Energy and Industrial Technology Development Organization (NEDO) of Japan
- CREST, Japan Science and Technology Agency
- Ministry of Education, Science, Sports and Culture
We report the synthesis and properties of oligonucleotides incorporating N(6)-[N-(pyren-1-ylmethyl) carbamoyl]deoxyadenosine (dA(pymcm)). We designed the ODN which incorporated two consecutive dA(pymcm) residues. It was revealed that on hybridization with the target DNA and RNA oligomers, the fluorescence spectra of ODNs having two consecutive dA(pymcm) molecules near the 5'-terminal position can change from the pyrene monomer emission to the excimer, depending on the chain length of the target DNA and RNA. These results indicated that dA(pymcm)-modified ODNs can be used as interesting hybridization sensors that are sensitive to the size of the target strand. (C) 2008 Elsevier Ltd. All rights reserved.
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