期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 16, 期 16, 页码 7592-7598出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2008.07.018
关键词
allergy; rat basophilic leukemia RBL-2H3 cells; polymethoxy flavone; degranulation; combinated treatment
Polymethoxy flavones (PMFs) are present in fruit tissues of Citrus species. It has been reported that flavonoids isolated from several Citrus have been shown to suppress the degranulation as inferred by histamine release in rat basophilic leukemia RBL-2H3 cells. In this study, we examined the effect of PMFs (PMF-1: 6,7,40,5'-tetramethoxy-5-monohydroxy flavone, PMF-2: 5,6,8,30,6'- pentamethoxy flavone, PMF-3: 5,6,7,30,40,5'-hexamethoxy flavone) on the degranulation in RBL-2H3 cells. All the PMFs suppressed the degranulation from Ag-stimulated RBL-2H3 cells. Interestingly, PMF-combination (PMF-1 + PMF-2; PMF-1 + PMF-3) treatment enhanced the inhibition of degranulation compared with PMF-single treatment. In order to clarify the inhibitory mechanism of degranulation by PMFs, we examined the activation of intracellular signaling molecules such as Lyn, Syk, and PLC gamma s. All the PMFs significantly suppressed the activation of Syk and PLC gamma s. In Ag-mediated activation of Fc epsilon RI on mast cells, three major subfamilies of mitogen-activated protein kinases, especially ERK44/42, were activated. These PMFs reduced the level of phospho-ERKs. The intracellular free Ca2+ concentration ([Ca2+]i) was elevated by Fc epsilon RI activation, and PMF treatment reduced the elevation of [Ca2+]i by suppressing Ca2+ in flux. Thus, it was suggested that the suppression of Ag-stimulated degranulation by these PMFs mainly is due to the Syk/PLC gamma s/PKC pathway and Ca2+ in flux. Furthermore, to be noted in the PMF-combination treatment, inactivation of Syk was enhanced compared with PMF-single treatment. But the inhibitory effect of degranulation by PMF-combination treatment was not associated with the suppression of Ca2+ in flux. (C) 2008 Elsevier Ltd. All rights reserved.
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