Pyrene-perylene as a FRET pair coupled to the N2 '-functionality of 2 '-amino-LNA

Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl NT-functionalized 2'-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 A apart to approximately 90% when the fluorophores were in close proximity. (C) 2007 Elsevier Ltd. All rights reserved.