期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 116, 期 6, 页码 969-976出版社
WILEY
DOI: 10.1002/jcb.25050
关键词
miR-540; ADIPOSE TISSUE-DERIVED STROMAL CELLS; ADIPOGENIC DIFFERENTIATION; PPAR-gamma
资金
- National Natural Science Foundation of China [81070802, 81271096]
- Ministry of Finance of P.R. China [2011SCUD4B14]
- National High Technology Research and Development Program of China [2011AA030107]
A better understanding of the molecular mechanisms in adipogenesis may provide new insights into adipose tissue-related diseases. Recently, microRNAs (miRNAs) have emerged as a class of epigenetic regulators of stem cell differentiation. In this study, we found that miR-540 was an essential negative regulator of adipogenic differentiation in adipose tissue-derived stromal cells (ADSCs). Lentivirus-mediated overexpression of miR-540 resulted in blockade of the expression of C/EBP-alpha and PPAR gamma, the two master transcription factors of adipogenesis, and deficient lipid accumulation, whereas inhibition of miR-540 promoted these processes. Target gen e reporter assays showed that miR-540 directly targeted the 3'-untranslated region (3'UTR) of PPAR gamma, resulting in a decrease of PPAR gamma protein expression. Collectively, these data suggest that miR-540 represents a new adipogenic inhibitor by, at least in part, targeting PPAR gamma. (C) 2015 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据