期刊
BIOMOLECULAR NMR ASSIGNMENTS
卷 5, 期 1, 页码 79-84出版社
SPRINGER
DOI: 10.1007/s12104-010-9272-3
关键词
Calbindin D-9k; 100% Complete assignment; Assignment strategy; NMR spectroscopy
Here we present the 100% complete assignment chemical shift of non-labile H-1, N-15 and C-13 nuclei of Calbindin D-9k P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysN zeta as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D-9k extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).
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