期刊
BIOMICROFLUIDICS
卷 8, 期 4, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4891599
关键词
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资金
- Defense Advanced Research Projects Agency (DARPA) N/MEMS S&T Fundamentals Program [N66001-1-4003]
- Space and Naval Warfare Systems Center Pacific (SPAWAR)
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [R01DK060957]
Cell filtration is a critical step in sample preparation in many bioapplications. Herein, we report on a simple, filter-free, microfluidic platform based on hydrodynamic inertial migration. Our approach builds on the concept of two-stage inertial migration which permits precise prediction of microparticle position within the microchannel. Our design manipulates equilibrium positions of larger microparticles by modulating rotation-induced lift force in a low aspect ratio microchannel. Here, we demonstrate filtration of microparticles with extreme efficiency (>99%). Using multiple prostate cell lines (LNCaP and human prostate epithelial tumor cells), we show filtration from spiked blood, with 3-fold concentration and >83% viability. Results of a proliferation assay show normal cell division and suggest no negative effects on intrinsic properties. Considering the planar low-aspect-ratio structure and predictable focusing, we envision promising applications and easy integration with existing lab-on-a-chip systems. (C) 2014 AIP Publishing LLC.
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