期刊
BIOMICROFLUIDICS
卷 8, 期 4, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4886771
关键词
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资金
- internal program MEF from the Fraunhofer Gesellschaft [125-600156]
- National Science Foundation (NSF) [DMR-1310266]
- Harvard MRSEC [DMR-0820484]
- RWTH Aachen University
- Fulbright Foundation
- Excellence Initiative, German federal government
- National Science Foundation under NSF Award [ECS-0335765]
- NSERC PGS D
A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
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