期刊
BIOMICROFLUIDICS
卷 7, 期 1, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4774311
关键词
bioMEMS; cellular biophysics; hydrodynamics; microchannel flow; tumours
资金
- MOST [2011CB933201, 2009CB930001, 2011CB707604]
- NSFC [51105086, 21025520, 90813032, 50890182]
- CAS [KJCX2-YW-M15, KJCX2-YW-H18]
In this work, we propose a rapid and continuous rare tumor cell separation based on hydrodynamic effects in a label-free manner. The competition between the inertial lift force and Dean drag force inside a double spiral microchannel results in the size-based cell separation of large tumor cells and small blood cells. The mechanism of hydrodynamic separation in curved microchannel was investigated by a numerical model. Experiments with binary mixture of 5- and 15-mu m-diameter polystyrene particles using the double spiral channel showed a separation purity of more than 95% at the flow rate above 30 ml/h. High throughput (2.5 x 10(8) cells/min) and efficient cell separation (more than 90%) of spiked HeLa cells and 20 x diluted blood cells was also achieved by the double spiral channel. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4774311]
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据