期刊
BIOMICROFLUIDICS
卷 6, 期 1, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3687409
关键词
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资金
- NSF [ECCS-1002255, ECCS-0736501]
- Intelligent Microsystems Center at Korea Institute of Science and Technology (KIST)
- National Research Council of Science & Technology (NST), Republic of Korea [2E2267] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
We propose a simple method for forming massive and uniform three-dimensional (3-D) cell spheroids in a multi-level structured microfluidic device by gravitational force. The concept of orienting the device vertically has allowed spheroid formation, long-term perfusion, and retrieval of the cultured spheroids by user-friendly standard pipetting. We have successfully formed, perfused, and retrieved uniform, size-controllable, well-conditioned spheroids of human embryonic kidney 293 cells (HEK 293) in the gravity-oriented microfluidic device. We expect the proposed method will be a useful tool to study in-vitro 3-D cell models for the proliferation, differentiation, and metabolism of embryoid bodies or tumours. (C) 2012 American Institute of Physics. [doi:10.1063/1.3687409]
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