期刊
BIOMICROFLUIDICS
卷 5, 期 2, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3580752
关键词
-
资金
- NIHR21 [AI087107]
- W.H. Coulter Foundation
- Center for Integration of Medicine and Innovative Technology under U.S. Army Medical Research [DAMD17-02-2-0006, W81XWH-07-2-0011, W81XWH-09-2-0001]
Embryonic stem cells (ESCs) are pluripotent with multilineage potential to differentiate into virtually all cell types in the organism and thus hold a great promise for cell therapy and regenerative medicine. In vitro differentiation of ESCs starts with a phase known as embryoid body (EB) formation. EB mimics the early stages of embryogenesis and plays an essential role in ESC differentiation in vitro. EB uniformity and size are critical parameters that directly influence the phenotype expression of ESCs. Various methods have been developed to form EBs, which involve natural aggregation of cells. However, challenges persist to form EBs with controlled size, shape, and uniformity in a reproducible manner. The current hanging-drop methods are labor intensive and time consuming. In this study, we report an approach to form controllable, uniform-sized EBs by integrating bioprinting technologies with the existing hanging-drop method. The approach presented here is simple, robust, and rapid. We present significantly enhanced EB size uniformity compared to the conventional manual hanging-drop method. (C) 2011 American Institute of Physics. [doi:10.1063/1.3580752]
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据