期刊
BIOMICROFLUIDICS
卷 5, 期 2, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3587095
关键词
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资金
- RASOR through BBSRC
- RASOR through EPSRC-GB
- Scottish Funding Council
- Chief Scientists' Office for Scotland
- Biotechnology and Biological Sciences Research Council [BB/C511572/1] Funding Source: researchfish
- Chief Scientist Office [SCD/04] Funding Source: researchfish
We discuss the ability to perform fluorescent immunocytochemistry, following cell fixation, using a microfluidic array of primary, nonadherent, single CD34+ stem cells. The technique requires small cell samples and proceeds with no cell loss, making it well-suited to monitoring these rare patient-derived cells. The chip allows us to correlate live cell dynamics across arrays of individual cells with post-translational modifications of intracellular proteins, following their exposure to drug treatments. Results also show that due to the microfluidic environment, the time scale of cell fixation was significantly reduced compared to conventional methods, leading to greater confidence in the status of the protein modifications studied. (C) 2011 American Institute of Physics. [doi:10.1063/1.3587095]
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