期刊
BIOMICROFLUIDICS
卷 5, 期 3, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3609264
关键词
biomedical optical imaging; bioMEMS; biomimetics; blood vessels; cellular biophysics; fluorescence spectroscopy; microfluidics; optical microscopy; surface chemistry; tissue engineering
资金
- Australian Research Council (ARC)
Microvascular network formation is a significant and challenging goal in the engineering of large three-dimensional artificial tissue structures. We show here the development of a fully patent, 3D endothelial cell (microvascular) microfluidic network that has a single inlet and outlet, created in only 28 h in a microdevice involving fluid flow equivalent to natural vasculature. Our microdevice features a tailored multi-rung ladder network, a stylized mimic of an arterial-to-venous pedicle, designed to also allow for systematic and reproducible cell seeding. Immunofluorescence staining revealed a highly contiguous endothelial monolayer (human umbilical vein endothelial cells) throughout the whole network after 24 h of continuous perfusion. This network persisted for up to 72 h of culture, providing a useful template from which the effects of surface chemistry, fluid flow, and environmental conditions on the development of artificial vascular networks ex vivo may be rapidly and robustly evaluated. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3609264]
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