期刊
BIOMICROFLUIDICS
卷 4, 期 1, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.3324847
关键词
bioelectric phenomena; biomembranes; cellular biophysics; DNA; proteins
资金
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0747886] Funding Source: National Science Foundation
In electroporation, an electric field transiently permeabilizes the cell membrane to gain access to the cytoplasm, and to deliver active agents such as DNA, proteins, and drug molecules. Past work suggests that the permeabilization is caused by the formation of aqueous, conducting pores on the lipid membrane, which are also known as electropores. The current-voltage relation across the membrane-bound pores is critical for understanding and predicting electroporation. In this work, we solve the Nernst-Planck equations in a geometry encompassing an isolated electropore to investigate this relation. In particular, we study cases where the intra- and extracellular electrical conductivities differ. We first derive an analytical solution, which is subsequently validated with a direct numerical simulation using a finite volume method. The main result of the current work is a formula for the effective pore resistance as a function of the pore radius, the membrane thickness, and the intra- and extracellular conductivities. This formula can be incorporated into whole-cell or planar-membrane electroporation models for system-level prediction and understanding.
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