4.5 Article

Gamma-Aminobutyric Acid A Receptor Subunit Expression and Cellular Localization in the Human Parkinsonian Globus Pallidus

期刊

WORLD NEUROSURGERY
卷 165, 期 -, 页码 E159-E168

出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.wneu.2022.05.121

关键词

Basal ganglia; GABA(A) receptor; Globus pallidus; Immunofluorescence; Parkinson disease; Receptor subunit

向作者/读者索取更多资源

The study aimed to characterize GABA(A) receptor subunit expression in the normal and parkinsonian human globus pallidus using fluorescence microscopy. The results showed no significant differences in GABA(A) receptor subunit expression between PD specimens and control specimens in the globus pallidus. Compensatory changes in GABA(A) receptor alpha 1 and alpha 3 subunit expression in response to PD-related signaling abnormalities in the globus pallidus were not observed in the PD cohort studied.
BACKGROUND: The gamma-aminobutyric acid A (GABA(A)) receptor is an important mediator of cellular signaling in the globus pallidus and might be implicated in the pathophysiology of Parkinson disease (PD). The goal of the present study was to characterize GABA(A) receptor subunit expression in the normal and parkinsonian human globus pallidus. METHODS: Postmortem brain specimens were obtained from 8 patients with pathological evidence of PD at autopsy and from 4 control patients without such evidence. These tissues were exposed to primary antibodies directed against the alpha 1 and alpha 3 subunits of the GABA(A) receptor and were visualized and quantified using fluorescence microscopy. RESULTS: No differences were found in the pallidal neuronal density in the control versus PD tissues. Projection neurons strongly expressed the alpha 1, alpha 3, and beta 2 GABA(A) receptor subunits. After normalizing the immunofluorescence intensities in the globus pallidus to those in the adjacent structures, no significant differences were found in GABA(A) receptor subunit expression in the globus pallidus between the PD specimens and the control specimens. CONCLUSIONS: Compensatory changes in GABA(A) receptor alpha 1 and alpha 3 subunit expression in response to PD-related signaling abnormalities in the globus pallidus did not occur in our PD cohort.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据