期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 19, 期 6, 页码 1400-1409出版社
WILEY
DOI: 10.1111/jcmm.12527
关键词
renalase; hypoxia-inducible factor; ischaemic preconditioning; ischaemia; reperfusion injury
资金
- National Natural Science Foundation of China [81100528]
- New-100 talent Plan of Shanghai Jiao Tong University School of Medicine
- Shanghai talents development fund
Ischaemic preconditioning (IPC) attenuates acute kidney injury (AKI) from renal ischaemia reperfusion. Renalase, an amine oxidase secreted by the proximal tubule, not only degrades circulating catecholamines but also protects against renal ischaemia reperfusion injury. Here, it has been suggested that the renoprotective effect of renal IPC is partly mediated by renalase. In a model of brief intermittent renal IPC, the increased cortex renalase expression was found to last for 48hrs. IPC significantly reduced renal tubular inflammation, necrosis and oxidative stress following renal ischaemia reperfusion injury. Such effects were attenuated by blocking renalase with an anti-renalase monoclonal antibody. We further demonstrated that renalase expression was up-regulated by hypoxia in vitro via an hypoxia-inducible factor (HIF)-1 mechanism. The IPC-induced up-regulation of renalase in vivo was also reduced by pre-treatment with an HIF-1 inhibitor, 3-(5-Hydroxymethyl-2-furyl)-1-benzyl indazole. In summary, the renoprotective effect of IPC is partly dependent on the renalase expression, which may be triggered by hypoxia via an HIF-1 mechanism. Endogenous renalase shows potential as a therapeutic agent for the prevention and treatment of AKI.
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