期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 191, 期 1, 页码 23-31出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.191.1.23
关键词
B cell development; precursor B cell receptor; lambda(5); c-kit; bcl-2
The capacity of precursor B (pre-B) I cells from fetal liver and bone marrow to proliferate and differentiate into surface immunoglobulin-positive immature B cells in vitro was analyzed. Both fetal liver- and bone marrow-derived progenitors do so in a pre-B cell receptor (pre-BCR)-dependent manner in tissue culture medium alone, without addition of other cells or cytokines. Approximately 20% of the initial pre-B I cells enter more than one division. Analyses at the single-cell level show that similar to 15% divide two to five times. Coculture of pre-B I cells with stromal cells did not enhance proliferation or differentiation, whereas the presence of interleukin 7, especially in combination with stromal cells, resulted mainly in the expansion of pre-B I cells and prevented their further differentiation Thus, the environment of fetal liver or bone marrow is not required for the pre-BCR to exert its function, which is to select and expand cells that have undergone an inframe V-H-D(H)J(H) rearrangement that produces a pre-BCR-compatible mu H chain. It appears unlikely that a ligand for the pre-BCR drives this pre-B cell proliferation.
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