期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 97, 期 1, 页码 477-482出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.97.1.477
关键词
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资金
- NHLBI NIH HHS [T32 HL007563, HL-32154, R01 HL055312] Funding Source: Medline
- NIGMS NIH HHS [P50 GM053789, GM-53789] Funding Source: Medline
- NINDS NIH HHS [R01 NS032385, NS32385] Funding Source: Medline
Although the function of metallothionein (MT), a 6- to 7-kDa cysteine-rich metal binding protein, remains unclear, it has been suggested from in vitro studies that MT is an important component of intracellular redox signaling, including being a target for nitric oxide (NO). To directly study the interaction between MT and NO in live cells, we generated a fusion protein consisting of MT sandwiched between two mutant green fluorescent proteins (GFPs). In vitro studies with this chimera (FRET-MT) demonstrate that fluorescent resonance energy transfer (FRET) can be used to follow conformational changes indicative of metal release from MT. Imaging experiments with live endothelial cells show that agents that increase cytoplasmic Ca2+ act via endogenously generated NO to rapidly and persistently release metal from MT, A role for this interaction in intact tissue is supported by the finding that the myogenic reflex of mesenteric arteries is absent in MT knockout mice (MT-/-) unless endogenous NO synthesis is blocked. These results are the first application of intramolecular green fluorescent protein (GFP)-based FRET in a native protein and demonstrate the utility of FRET-MT as an intracellular surrogate indicator of NO production, In addition, an important role of metal thiolate clusters of MT in NO signaling in vascular tissue is revealed.
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