期刊
ACTA TROPICA
卷 74, 期 1, 页码 89-93出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0001-706X(99)00054-6
关键词
human cutaneous leishmaniasis; Leishmania sp.; mini-exon gene; spliced leader; characterization; monoclonal antibodies; PCR; hybridization
Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmanin-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOMIBR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent. (C) 2000 Elsevier Science B.V. All rights reserved.
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