期刊
FEBS LETTERS
卷 465, 期 1, 页码 2-7出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(99)01712-3
关键词
meprin; astacin family; expression; laminin cleavage; Baculovirus
Meprins are astacin-like metalloproteases of renal and intestinal epithelia and embryonic neuroepithelial cells. The full length cDNA of the human meprin alpha subunit has been overexpressed in baculovirus-infected insect cells yielding the tetrameric proprotein which could be proteolytically activated and affinity-purified to homogeneity. Recombinant meprin a hydrolyzes the synthetic substrate N-benzoyl-tyrosyl-p-amino-benzoic acid (PABA-peptide) and cleaves by limited proteolysis the basement membrane constituents laminin 1 and laminin 5. This supports a concept that meprin alpha, when basolaterally secreted by human colon carcinoma epithelial cells, increases the proteolytic capacity for tumor progression in the stroma. (C) 2000 Federation of European Biochemical Societies.
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