期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1483, 期 2, 页码 275-284出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1388-1981(99)00194-8
关键词
cotton fiber; lipid transfer protein; gene family; alloallele; Gossypium hirsutum
There are six to eight lipid transfer protein-related genes in the allotetraploid Gossypium hirsutum genome, three of which, FSltp1, FSltp2 and FSltp3, were isolated in two genomic clones. All three genes potentially encode full-length lipid transfer proteins (LTPs), but sequence differences between these and the cotton fibre-specific cDNA previously reported, pFS6 suggest that none is likely to encode the fibre transcripts. Gene-specific RT-PCR experiments showed that expression patterns of the three new LTP genes are similar to each other, with transcripts present in all aerial cotton tissues tested, and only pFS6 transcripts are present in fibre cells at elevated levels. PCR analysis of cotton genomic DNA suggested that FSltp1 and FSltp3 are alloalleles, with FSltp3 being derived from the ancestral A genome, and FSltp1 from the D genome. The fibre-specific gene, yet to be isolated, and FSltp2 are also derived from the ancestral D genome of G. hirsutum. (C) 2000 Elsevier Science B.V. All rights reserved.
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