期刊
BIOMETALS
卷 25, 期 5, 页码 939-949出版社
SPRINGER
DOI: 10.1007/s10534-012-9561-7
关键词
Chromate reduction; Photosynthesis; Rhodobacter sphaeroides; Two-dimensional gel electrophoresis; Atomic force microscopy; Attenuated total reflection-fourier transformed infrared spectroscopy
资金
- Italian Ministry of Research Education and Education
- COST Action [CM0902]
The response of the carotenoidless Rhodobacter sphaeroides mutant R26 to chromate stress under photosynthetic conditions is investigated by biochemical and spectroscopic measurements, proteomic analysis and cell imaging. Cell cultures were found able to reduce chromate within 3-4 days. Chromate induces marked changes in the cellular dimension and morphology, as revealed by atomic force microscopy, along with compositional changes in the cell wall revealed by infrared spectroscopy. These effects are accompanied by significant changes in the level of several proteins: 15 proteins were found up-regulated and 15 down-regulated. The protein content found in chromate exposed cells is in good agreement with the biochemical, spectroscopic and microscopic results. Moreover at the present stage no specific chromate-reductase could be found in the soluble proteome, indicating that detoxification of the pollutant proceeds via aspecific reductants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据