期刊
JOURNAL OF BIOTECHNOLOGY
卷 77, 期 1, 页码 103-114出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1656(99)00210-2
关键词
NMR; complex mixture; transgenic crops; liquid chromatography; fingerprinting; substantial equivalence; comparison; food safety
A common element in designed guidelines for assessment of the food safety of transgenic crops is centred on a comparative analytical analysis with conventionally bred crop plants, assuming that these products have a long history of safe use (i.e. OECD-principle of substantial equivalence). Tn this study we examine the utility of an off-line combination of 400 MHz proton (H-1)-NMR spectroscopy and liquid chromatography (LC) for the multi-component comparison of low-molecular weight compounds (i.e. chemical fingerprinting) in complex plant matrices. The developed NMR-methodology can contribute to the demonstration of substantial equivalence by its ability to compare possible compositional alterations in a novel food crop with respect to related non-transgenic reference lines. In this respect a hierarchical approach is proposed by comparing the chemical fingerprints of the transgenic crop plant to those of: (1) isogenic parental or closely related lines bred at identical and multiple sites; (2) extended ranges of commercial varieties of that plant: and (3) downstream processing effects. This is of importance to assess the likelihood that some of the statistical differences in a transgenic crop plant may be false positives due to chance alone or arose from natural genetic and/or physiologic variations. (C) 2000 Elsevier Science B.V. All rights reserved.
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