期刊
FOOD CHEMISTRY
卷 68, 期 3, 页码 309-313出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0308-8146(99)00196-X
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Aflatoxin B-1 (AFB(1)), extracted from Aspergillus flavus ATCC 15517, was detoxified up to 60% by commercial horse radish peroxidase (HRP) (200 U/mg) in vitro. Partially purified peroxidase enzymes from freshly harvested radish root having 20 and 30 U/mg of protein were made to act upon the toxin in vitro and showed approximately 30 and 38% conversion, respectively. The optimum enzymatic reaction occurred in 50 mM phosphate buffer at 20 degrees C, pH6, incubation time 60 min and normal pressure. Hydrogen peroxide in 5 mM concentration was used as oxidising agent in all the reactions performed. The reaction product was tested for its toxicity on Bacillus megaterium and other micro-organisms. Toxicity tests resulted in 97 and 30% growth of bacilli when 200 and 30 U/mg enzyme was used for the reaction, respectively. (C) 1999 Elsevier Science Ltd. All rights reserved.
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