期刊
JOURNAL OF CELL SCIENCE
卷 129, 期 1, 页码 155-165出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.178293
关键词
NEET proteins; NAF-1; Mitochondria; ROS; Apoptosis; Cancer
类别
资金
- Israel Science Foundation [ISF 865/13]
- University of North Texas College of Arts and Sciences
- National Science Foundation [PHY-1427654, MCB-1214457]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM101467] Funding Source: NIH RePORTER
- Direct For Mathematical & Physical Scien [1427654] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience [1214457] Funding Source: National Science Foundation
Maintaining iron (Fe) ion and reactive oxygen species homeostasis is essential for cellular function, mitochondrial integrity and the regulation of cell death pathways, and is recognized as a key process underlying the molecular basis of aging and various diseases, such as diabetes, neurodegenerative diseases and cancer. Nutrient-deprivation autophagy factor 1 (NAF-1; also known as CISD2) belongs to a newly discovered class of Fe-sulfur proteins that are localized to the outer mitochondrial membrane and the endoplasmic reticulum. It has been implicated in regulating homeostasis of Fe ions, as well as the activation of autophagy through interaction with BCL-2. Here we show that small hairpin (sh) RNA-mediated suppression of NAF-1 results in the activation of apoptosis in epithelial breast cancer cells and xenograft tumors. Suppression of NAF-1 resulted in increased uptake of Fe ions into cells, a metabolic shift that rendered cells more susceptible to a glycolysis inhibitor, and the activation of cellular stress pathways that are associated with HIF1 alpha. Our studies suggest that NAF-1 is a major player in the metabolic regulation of breast cancer cells through its effects on cellular Fe ion distribution, mitochondrial metabolism and the induction of apoptosis.
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