期刊
NATURE BIOTECHNOLOGY
卷 18, 期 2, 页码 163-167出版社
NATURE AMERICA INC
DOI: 10.1038/72608
关键词
trehalose; alpha-toxin; cryopreservation; fibroblasts; keratinocytes
资金
- NIDDK NIH HHS [DK46270] Funding Source: Medline
- NINDS NIH HHS [NS26760] Funding Source: Medline
We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus alpha-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.
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