期刊
CELL DEATH AND DIFFERENTIATION
卷 7, 期 2, 页码 197-206出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.cdd.4400595
关键词
apoptosis; caspase; camptothecin; ceramide; etoposide; peripheral blood lymphocytes
The effect of etoposide acid camptothecin, two topoisomerase inhibitors directed against topoisomerases II and I, respectively, was evaluated on human peripheral blood lymphocytes, Etoposide and camptothecin induced apoptosis of mitogen-activated but not resting CD4(+) and CD8(+) T lymphocytes. Cell sensitivity to these agents required G(1) to S-phase transition of the cell cycle. Conversely, daunorubicin, an intercalating agent and topoisomerase II inhibitor, induced apoptosis of both resting and activated lymphocytes, Although etoposide and camptothecin induced CD95-ligand mRNA expression, drug-induced apoptosis of activated human lymphocytes was not inhibited by CD95 antagonists. Drug-induced cell death was also not inhibited by p55 TNFR-Ig fusion protein. Activation of the caspases cascade was suggested by the partial inhibitory effect of the tripeptide zVAD-fmk and documented by activation of caspase 3, Finally etoposide and camptothecin induced a rapid production of ceramide in activated but not resting peripheral blood lymphocytes, suggesting that ceramide might initiate the signaling apoptotic cascade in sensitive cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据