期刊
JOURNAL OF CELL SCIENCE
卷 128, 期 4, 页码 804-814出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.164004
关键词
IGF1R; Endometrium; Implantation; Embryo; miR-145; miRNA
类别
资金
- University of Manchester
- Society for Endocrinology
- Tommy's the Baby Charity
- Action Research Endowment Fund
- Manchester Biomedical Research Centre
- Greater Manchester Comprehensive Local Research Network
- Biotechnology and Biological Sciences Research Council [BB/J021636/1] Funding Source: researchfish
- Medical Research Council [G0700092, G0300484, G0801057, 1543095] Funding Source: researchfish
- Wellbeing of Women [RG1442] Funding Source: researchfish
- BBSRC [BB/J021636/1] Funding Source: UKRI
- MRC [G0700092, G0801057, G0300484] Funding Source: UKRI
Successful implantation requires the synchronization of viable embryonic development with endometrial receptivity. The mechanisms allowing for the initiation of crosstalk between the embryo and the endometrium remain elusive; however, recent studies have revealed that there are alterations in endometrial microRNAs (miRs) in women suffering repeated implantation failure and that one of the altered miRs is miR-145. We assessed the role of miR-145 and its target IGF1R, in early implantation. miR-145 overexpression and IGF1R knockdown were achieved in Ishikawa endometrial cells. Quantitative PCR, western blotting and 3'UTR luciferase reporter assays confirmed that IGF1R is a direct target of miR-145 in the endometrium. Attachment of mouse embryos or IGF1-coated beads to endometrial epithelial cells was used to study the effects of altered miR-145 and/or IGF1R expression on early implantation events. miR-145 overexpression or specific reduction of IGF1R impaired attachment in both cases. An IGF1R target protector prevented the miR-145-mediated reduction in IGF1R and reversed the effect of miR-145 overexpression on attachment. The data demonstrate that miR-145 influences embryo attachment by reducing the level of IGF1R in endometrium.
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