Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines

Anther derived spontaneous doubled haploid (DH-R-1) plants were developed from an intercultivar F-1 hybrid (Holland) of type pepper cv. (Capsicum annuum L. var. annuum, 2n = 24). Regenerants were grown in greenhouses for seed production to R-2-generation (DH-R-2) prior to flow cytometric analysis which revealed DH-R-1 plants with haploid (n = 12); doubled haploid, DH, (2n = 24); tetraploid (2n = 48); and aneuploid genomes. Pollen grains of anther cultures showed a high decrease in viability during the first two weeks of incubation time. The DNA samples of the 47 DH-R-2 individuals were bulked in nine groups and subjected to PCR analysis for estimating the level of homogeneity versus heterogeneity in comparison to the control of anther donor bulk of five plants of F-1 hybrid pepper cv. (DH-R-0). in total 26 primers were applied, 22 of them revealed scorable PCR profiles with 54 scorable PCR bands. Of these, 19 (35.2%) bands generated by 7 primers were polymorphic. All of the nine bulks of DH-R-2 plane were discriminated at least by one primer.