期刊
JOURNAL OF ECONOMIC ENTOMOLOGY
卷 93, 期 1, 页码 26-30出版社
ENTOMOL SOC AMER
DOI: 10.1603/0022-0493-93.1.26
关键词
Ostrinia nubilalis; F-2 screen; transgenic corn; resistance management
类别
The refuge plus high-dose strategy for resistance management assumes that the frequency of resistance alleles is low. We used an F-2 screen to estimate the frequency of resistance to transgenic corn that produces Bacillus thuringiensis Berliner Cry1Ab toxin (Bt corn) in an Iowa population of European corn borer, Ostrinia nubilalis (Hubner). We also proposed a modification to the statistical analysis of the F-2 screen that extends its application for nonuniform prior distributions and for repeated sampling of a single population. Based on a sample of 188 isofemale lines derived from females caught at light traps during the 2nd flight of 1997, we show with 95% confidence that the frequency of resistance to Bt corn was <3.9 x 10(-3) in this Iowa population. These results provide weak evidence that the refuge plus high-dose strategy may be effective for managing resistance in O. nubilalis to Bt corn. Partial resistance to Cry1Ab toxin was found commonly. The 95% CI for the frequency of partial resistance were [8.2 x 10(-4), 9.4 x 10(-3)] for the Iowa population. Variable costs of the method were $14.90 per isofemale line. which was a reduction of 25% compared with our initial estimate.
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